synth & diy bio :: Remember, remember, the velvet November


node:	synth & diy bio
template:	3
parent:	Empire State of Sabotage Embassy
owner:	pht
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created:	18.04.2019 - 18:56:33
updated:	19.04.2019 - 22:59:41

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8617850

ABSOLUT
KYBERIA

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5c26a291-de44-4718-862a-ffd8765a3373_RIKILT_tech-01_fe105cb3_530x372.png

https://en.wikipedia.org/wiki/Synthetic_biology

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Foldscope is the ultra-affordable paper microscope. It was designed to be portable and durable, while performing on par with conventional research microscopes (140X magnification and 2 micron resolution).

As a company, Foldscope Instruments Inc’s mission is to break down the price barrier between people & the curiosity and excitement of scientific exploration!

Through the purchase of our products, you directly support our mission and enable us to bring tools to communities around the world.
https://www.foldscope.com
https://en.wikipedia.org/wiki/Foldscope

Niečo trochu viac advanced:
https://www.planktonscope.org

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dostal som to od kamosa, medzi tym som si ale kupil aj normalny mikroskop tak mozem niekomu v BA posunut ak by bol zaujem...

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a poštou poslať do Žiliny? :)

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a co by si s tym stvaral?

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pozeral so synom malý svet

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Posli do posty adresu

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toto tu uz bolo tak 5 rokov dozadu, nie je to az tak originalny napad:

1$ mikroskop: https://www.youtube.com/watch?v=vQJDV4GE4aY

edit: prave pozeram, ze to je ta ista firma :)

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mal by niekto zaujem, pozdielat shipping costs napr cez shipito ?

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https://www.inaturalist.org/
https://en.wikipedia.org/wiki/INaturalist

toto je moc pekne, na pozorovanie a identifikaciu druhov 'shazaam for nature', a je to neziskova vec ziaden startup bs
https://www.inaturalist.org/observations?place_id=44569

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All things Biohacking.

zatial minimalisticky topic. Hadam sa casom rozsiri.

FB skupina, ktoru uz nejaky cas sledujem: Bio Hacker Tribe

Celkom pekna mapa: Why Nootropics?

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In vivo production of psilocybin in E. coli
https://www.sciencedirect.com/science/article/pii/S109671761930309X

Highlights
• First example of psilocybin production in prokaryotic host.

• Genetic optimization shows high sensitivity to changes in transcriptional landscape.

• Pathway bottlenecks removed through fine-tuned optimization.

• Gram-scale production of psilocybin in Escherichia coli.

Abstract
Psilocybin, the prodrug of the psychoactive molecule psilocin, has demonstrated promising results in clinical trials for the treatment of addiction, depression, and post-traumatic stress disorder. The development of a psilocybin production platform in a highly engineerable microbe could lead to rapid advances towards the bioproduction of psilocybin for use in ongoing clinical trials. Here, we present the development of a modular biosynthetic production platform in the model microbe, Escherichia coli. Efforts to optimize and improve pathway performance using multiple genetic optimization techniques were evaluated, resulting in a 32-fold improvement in psilocybin titer. Further enhancements to this genetically superior strain were achieved through fermentation optimization, ultimIn vivo production of psilocybin in E. coliately resulting in a fed-batch fermentation study, with a production titer of 1.16 g/L of psilocybin. This is the highest psilocybin titer achieved to date from a recombinant organism and a significant step towards demonstrating the feasibility of industrial production of biologically-derived psilocybin.

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rob rhinehart zije! (po tom co si odstranil crevny mikrobiom a zahadne odisiel z internetu aj rosa labs)

Investing in the Future of Food with Rob Rhinehart and Brian Frank
https://www.spreaker.com/user/10197011/investing-in-the-future-of-food-with-rob

Brian Frank (@bfrank) of FTW Ventures, and Rob Rhinehart (@robrhinehart) of Soylent, join Erik on this episode.

They discuss:

- Some of the most exciting developments in bioscience these days.

- The possibilities for biohacking and whether there is an “AWS for bioscience” emerging.

- The interplay of startups and incumbents in the space and how incumbents can be more helpful to the startup community.

- What is under- or over-hyped in the space.

- The existing big companies in food and bioscience and what will change in the next 5-10 years.

- The future of retail and how data can transform supply chains.

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v piatok 22. 11. v paralelnej polis
https://www.facebook.com/events/1473050229500557/
Rozhodli sme sa zorganizovať neformálne stretnutia v úzkom kruhu nadšencov vedy. Na stretnutiach by sme sa radi venovali rôznym témam zo sveta biotechu a microbio.
Bude to taký ten typ neformálneho rozhovoru o vede, živote a biznise pri pohári dobrého vína alebo piva (optional).
Na BioWine nájdete parťákov, s ktorými takto viete stráviť príjemný “bio” večer :)

Na to, aby si sa mohol zúčastniť nemusíš byť odborník, stačí ti túžba spoznávať. Preferenčne je však event určený pre vedcov alebo študentov microbio odborov. Tešíme sa na teba!

P.S. Zabezpečíme nejaké nápoje, ale feel free priniesť čokoľvek, čo máš rád.

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diy bio ba telegram group
https://t.me/diybiobratislava

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Venuje sa tu niekto pestovaniu rias?

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Founders of nonfood, Lucy Chinen & Sean Raspet discuss contemporary food supply chains and sustainable food futures, including the R&D of their own algae-based nonbar. Along the way, we address: monocultures, fear, skeuomorphic flavor, cellular agriculture, and the real cost of "all-natural." (w/ Caroline Busta, Daniel Keller, @LILINTERNET)

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How biologists are creating life-like cells from scratch
https://www.nature.com/articles/d41586-018-07289-x
http://buildacell.io/
http://buildacell.io/engineering/build-a-cell/

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https://soundcloud.com/a16z/damage-free-genome-editing-next-in-crispr-recent-journal-papers

Two recent scientific journal papers show what’s possible when CRISPR moves from cutting DNA tool to a full-fledged platform — expanding its toolkit for medicine across R&D, therapeutics, and diagnostics:

“Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration” in Nature — by Sanne Klompe, Phuc Vo, Tyler Halpin-Healy, and Samuel Sternberg (of Columbia University)

“RNA-guided DNA insertion with CRISPR-associated transposases” in Science — by Jonathan Strecker, Alim Ladha, Zachary Gardner, Jonathan Schmid-burgk, Kira Makarova, Eugene Koonin, and Feng Zhang (of the Broad Institute)

What do these two papers — both about techniques for getting rid of the need to cut the genome to edit it — make possible going forward, given the ongoing shift of biology becoming more like engineering? Where are we in the wave of the genome engineering “developer community” building on top of CRISPR with a constantly growing suite of programmable functionalities? a16z bio general partner Jorge Conde and bio deal team partner Andy Tran chat with Hanne Tidnam about these trends — and these two papers — in this short internal hallway-style conversation, part of our new a16z Journal Club series.

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Illustrated Guide to Home Biology Experiments: All Lab, No Lecture (DIY Science)

Experience the magic of biology in your own home lab. This hands-on introduction includes more than 30 educational (and fun) experiments that help you explore this fascinating field on your own. Perfect for middle- and high-school students and DIY enthusiasts, this full-color guide teaches you the basics of biology lab work and shows you how to set up a safe lab at home.

The Illustrated Guide to Home Biology Experiments is also written with the needs of homeschoolers firmly in mind, as well as adults who are eager to explore the science of nature as a life-long hobby. To get the most from the experiments, we recommend using this guide in conjunction with a standard biology text, such as the freely downloadable CK-12 Biology (ck-12.org).

Master the use of the microscope, including sectioning and staining
Build and observe microcosms, soda-bottle worlds of pond life
Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA
Extract, isolate, and observe DNA
Explore photosynthesis, osmosis, nitrogen fixation, and other life processes
Investigate the cell cycle (mitosis and cytokinesis)
Observe populations and ecosystems, and perform air and water pollution tests
Investigate genetics and inheritance
Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection
Gain hands-on lab experience to prepare for the AP Biology exam
https://www.thehomescientist.com/manuals/Illustrated_Guide_to_Home_Biology_Experiments.pdf

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uplne prve helloworld pokusy na agare

vzorka z drazdiaku po 1 dni:

po tyzdni+:

aspon 5 roznych kolonii

odtlacok zubnej kefky po 3 dnoch:

asi vydareny klon bez kontaminacie

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neviem ake medium pouzivas (predpokladam ze variant domaceho LB), ale Drazdiak aj ine priklady mozu byt uplne nevypovedne, lebo kopa mikroorganizmov nie je tak jednoducho kultivovatelna a potrebuju specialnejsie podmienky

niekde by som mala mat pdfko s najcastejsimi mikroorganizmami, ktore pripravil Virologicky ustav na air sampling pri Noci vyskumnikov, zopar veci co ti vyrastlo by si tam urcite identifikoval :)

edit:

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wow dik
pouzil som potato dextrose agar

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domaci LB nieje zbytocne zlozity? (co pozeram nejake zlozenia)
ja som kupil off-the-shelf malt extract agar s peptonom, ale chcem si robit aj svoj

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to mas to iste:
nejaky extract - malt (v pripade LB yeast)
nejake stiepene bielkoviny, ako zdroj aminokyselin - pepton (v pripade LB tryptone)
agar
dosolis (NaCl), povaris a podavas :)

zavisi co chces kultivovat - pozeram ze ten malt je general na yeast & fungi

aww: https://commons.wikimedia.org/wiki/File:Colony_morphology.svg

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kebyze to nieje off the shelf tak do toho malt extract agaru davam nutritional yeast. nejaky pepton alebo trypton neviem kde by som zhanal :)
(a hej, klonujem na tom huby)

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naco treba dosolit? nie je prave sol antibakterialna?

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zlozkou media je sol, okrem toho ze sluzi ako zdroj Na/Cl aj kvoli osmotickemu tlaku
https://en.wikipedia.org/wiki/Lysogeny_broth

vysoka koncentracia soli ano (ak sa nebavime o halofiloch)
fyziologicka koncentracia resp. *urcity* rozsah koncentracii pre *urcite* organizmy nie

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drazdiak po dalsom tyzdni:
HGxXVcI.jpg?1

po dychnuti a +1 tyzdni:

uz zdrha:

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my sme skusili 2 days ago, uz je tam prvy feši

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Na čo sa to pozerám?
Petriho misku poznám. :-)

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neviem na co sa ty pozeras, ale na obrazku su nejake random neidentifikovane bakterialne kolonie a mycelium z kusku hypsizygus tessellatus

axone Biohacking
axone forumz