10 g of any lysergic acid


amide from various natural sources dissolved in 200 ml of methanolic


KOH solution and the methanol removed immediately in vacuo. The residue


is treated with 200 ml of an 8% aqueous solution of KOH and the mixture


heated on a steam bath for one hour. A stream of nitrogen gas is passed


through the flask during heating and the evolved NH3 gas may be titrated


is HCl to follow the reaction. The alkaline solution is made neutral


to congo red with tartaric acid, filtered, cleaned by extraction with


ether, the aqueous solution filtered and evaporated. Digest the MeOH


to remove some of the coloured material from the crystals of lysergic


acid.

Arrange the lighting


in the lab similarly to that of a dark room. Use photographic red and


yellow safety lights, as lysergic acid derivatives are decomposed when


light is present. Rubber gloves must be worn due to the highly poisonous


nature of ergot alkaloids. A hair drier, or, better, a flash evaporator,


is necessary to speed up steps where evaporation is necessary.

2. Syntéza LSD isomeru


z kyseliny lysergové

Step I. Use Yellow light






5.36 g of d-lysergic acid are suspended in 125 ml of acetonitrile and


the suspension cooled to about -20 C in a bath of acetone cooled with


dry ice. To the suspension is added a cold (-20 C) solution of 8.82


g of trifluoroacetic anhydride in 75 ml of acetonitrile. The mixture


is allowed to stand at -20 C for about 1.5 hours during which the suspended


material dissolves, and the d-lysergic acid is converted to the mixed


anhydride of lysergic and trifluoroacetic acids. The mixed anhydride


can be separated in the form of an oil by evaporating the solvent in


vacuo at a temperature below 0 C, but this is not necessary. Everything


must be kept anhydrous.

Step II. Use Yellow light






The solution of mixed anhydrides in acetonitrile from Step I is added


to 150 ml of a second solution of acetonitrile containing 7.6 g of diethylamine.


The mixture is held in the dark at room temperature for about 2 hours.


The acetonitrile is evaporated in vacuo, leaving a residue of LSD-25


plus other impurities. The residue is dissolved in 150 ml of chloroform


and 20 ml of ice water. The chloroform layer is removed and the aqueous


layer is extracted with several portions of chloroform. The chloroform


portions are combined and in turn washed with four 50 ml portions of


ice-cold water. The chloroform solution is then dried over anhydrous


Na2SO4 and evaporated in vacuo.

Vzniklo LSD s prímesí


isomeru, zpusobujících bolesti hlavy, nevolnost a další nežádoucí efekty.


Psychoaktivní je pouze d-stereoizomer. Nutno provést purifikaci podle


bodu 3.

3. Oddelení cistého


LSD od isomeru

Step I. Use darkroom and


follow with a long wave UV



The material is dissolved in a 3:1 mixture of benzene and chloroform.


Pack the chromatography column with a slurry of basic alumina in benzene


so that a 1 inch column is six inches long. Drain the solvent to the


top of the alumina column and carefully add an aliquot of the LSD-solvent


solution containing 50 ml of solvent and 1 g LSD. Run this through the


column, following the fastest moving fluorescent band. After it has


been collected, strip the remaining material from the column by washing


with MeOH. Use the UV light sparingly to prevent excessive damage to


the compounds. Evaporate the second fraction in vacuo and set aside


for Step II. The fraction containing the pure LSD is concentrated in


vacuo and the syrup will crystallise slowly. This material may be converted


to the tartrate by tartaric acid and the LSD tartrate conveniently crystallised.


MP 190-196 C.

Step II. Use Red light



Dissolve the residue derived from the methanol stripping of the column


in a minimum amount of alcohol. Add twice that volume of 4 N alcoholic


KOH solution and allow the mixture to stand at room temperature for


several hours. Neutralise with dilute HCl, make slightly basic with


NH4OH and extract with chloroform as in #2. Evaporate in vacuo and chromatograph


as in the previous step.

Note: Lysergic acid compounds


are unstable to heat, light and oxygen. In any form it helps to add


ascorbic acid as an anti- oxidant, keeping the container tightly closed,


light-tight with aluminum foil, and in a refrigerator.

N-acetyl-LSD : známé


jako ALD-52, horší než LSD, zhruba 90% úcinnost, mírné potlacení


nervové aktivity, klidnejší, méne intenzivní prubeh, nekdy je na tripech


místo LSD

N-methyl-LSD : lepší


než klasické LSD, dokonalejší pusobení na nervovou soustavu